Anti-Cleaved-Caspase-7 p20 antibody
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概述
- 产品描述This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded protein is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves and activates sterol regulatory element binding proteins (SREBPs). Proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-|-Gly-217' bond. Overexpression promotes programmed cell death. Cleavages by granzyme B or caspase-10 generate the two active subunits. Propeptide domains can also be cleaved efficiently by caspase-3. Active heterodimers between the small subunit of caspase-7 and the large subunit of caspase-3, and vice versa, also occur. Belongs to the peptidase C14A family. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed
- 产品名称Anti-Cleaved-Caspase-7 p20 antibody
- 分子量20 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,IF,IHC-P
- 抗体类型兔多抗
- 免疫原The antiserum was produced against synthesized peptide derived from human Caspase 7. AA range:149-198
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/ml
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 亚型IgG
- 纯化方式The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- 亚细胞定位Cytoplasm.
- 其它名称Caspase 7 subunit p20 antibody
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应用
WB: 1:500-1:2000
IF: 1:50-1:200
IHC-P: 1:50-1:200
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Fig1: Western blot analysis of Cleaved-Caspase-7 p20 on Jurkat cells lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibodywas used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
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