Anti-TMEFF2 antibody

Anti-TMEFF2 antibody

• 概述

• 产品描述May be a survival factor for hippocampal and mesencephalic neurons. The shedded form up-regulates cancer cell proliferation, probably by promoting ERK1/2 phosphorylation.
• 产品名称Anti-TMEFF2 antibody
• 分子量37 KDa
• 种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Guinea Pig
• 验证应用WB,IHC-P
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human TMEFF2 301-374/374
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Isoform 1: Membrane; Single-pass type I membrane protein (Potential). Isoform 2: Membrane; Single-pass type I membrane protein (Potential). Isoform 3: Secreted.
• 其它名称Tomoregulin-2
  HPP 1
  HPP1
  Hyperplastic polyposis protein 1
  TEFF 2
  TEFF2
  TENB 2
  TENB2
  Tomoregulin
  TPEF
  TR
  Transmembrane protein containing epidermal growth factor and follistatin domains
  Transmembrane protein TENB2
  Transmembrane protein with EGF like and two follistatin like domains 2
  TEFF2_HUMAN.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Paraformaldehyde-fixed, paraffin embedded (rat spinal tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TMEFF2) Polyclonal Antibody, Unconjugated ) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (TMEFF2) Polyclonal Antibody, Unconjugatedat 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig3: Sample:
  Cerebrum (Rat) Lysate at 40 ug
  Primary: Anti-TMEFF2at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 37 kD
  Observed band size: 48 kD

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！