Anti-UFD1L antibody [F3-A6]

Anti-UFD1L antibody [F3-A6]

• 概述

• 产品描述Ubiquitin-mediated proteolysis requires the transfer of ubiquitin (Ub) to lysine groups on selected cellular proteins, which then potentiates the proteolytic degradation of these protein conjugates by the 26S proteasome. Ub-fusions are cleaved by Ub-specific processing proteases (UBps) or alternatively by the Ub-fusion degradation (UFD) pathway. The UBP pathway targets the C-terminal glycine residue on Ub that is involved in the formation of Ub-conjugates, while UFD proteins preferentially cleave Ub-conjugated proteins that contain an amino acid substitution at this glycine residue. The UFD1 protein was originally characterized in the yeast S. cerevisiae and subsequently, the human homolog UFD1 or UFD1L was identified. In vitro, UFD1 attenuates the degradation of Ub-fusions, which have a proline or valine residue substituted at the Gly76 moiety, by the selective multiubiquitination of the Ub chain of the Ub-conjugate. Mutations within the UFD1 gene are implicated in the development of CATCH22 syndrome, which is characterized by cardiac defects, cleft palate and hypocalcemia, suggesting that this proteolytic pathway may be involved in the progression of these developmental defects.
• 产品名称Anti-UFD1L antibody [F3-A6]
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2b
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus. Cytoplasm?.
• 其它名称
  • UB fusion protein 1 antibody
  • Ubiquitin fusion degradation 1 like (yeast) antibody
  • Ubiquitin fusion degradation 1 like antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:200

•  

  

  Fig1: Western blot analysis of UFD1L on human UFD1L recombinant protein using anti-UFD1L antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of UFD1L on HEK293 (1) and UFD1L-hIgGFc transfected HEK293 (2) cell lysate using anti-UFD1L antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of UFD1L on different cell lysate using anti-UFD1L antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: K562
  Lane 2: Hela
  Lane 3: A431
  Lane 4: PC-2
  Lane 5: A549

   

  

  Fig4: ICC staining UFD1L (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-UFD1L antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-UFD1L antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of Hela cells with UFD1L antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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