Anti-Serotonin transporter antibody

Anti-Serotonin transporter antibody

• 概述

• 产品描述Serotonin transporter whose primary function in the central nervous system involves the regulation of serotonergic signaling via transport of serotonin molecules from the synaptic cleft back into the pre-synaptic terminal for re-utilization. Plays a key role in mediating regulation of the availability of serotonin to other receptors of serotonergic systems. Terminates the action of serotonin and recycles it in a sodium-dependent manner.
• 产品名称Anti-Serotonin transporter antibody
• 分子量70 KDa
• 种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Sheep,Guinea Pig
• 验证应用WB,IHC-P
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human 5-HTT 401-500/630
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Cell membrane; Multi-pass membrane protein. Endomembrane system; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein.
• 其它名称5HT transporter
  5-HTT
  5-HTTLPR
  5HTT
  hSERT
  HTT
  OCD1
  SERT
  SERT1
  SC6A4_HUMAN
  SERT
  SLC6A4
  Sodium dependent serotonin transporter
  Sodium-dependent serotonin transporter
  Solute carrier family 6 member 4
  sodium-dependent serotonin transporter
  5-hydroxytryptamine transporter
  5HT transporter
  Na+/Cl- dependent serotonin transporter.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Sample:
  Cerebrum (Mouse) Lysate at 40 ug
  Primary: Anti-SLC6A4at 1/500 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 70 kD
  Observed band size: 70 kD

   

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Serotonin transporter) Polyclonal Antibody, Unconjugatedat 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

   

  

  Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (Serotonin transporter) Polyclonal Antibody, Unconjugatedat 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.

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