Anti-ox-LDL antibody

Anti-LDL antibody

• 概述

• 产品名称Anti-ox-LDL antibody
• 分子量31 KDa
• 种属反应性Human
• 验证应用WB,IHC-P,FC
• 抗体类型兔多抗
• 免疫原Full length protein from human plasma
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Secreted (probable)
• 其它名称ox-LDL: LDL (Copper oxidized)
  Cu2SO4 oxidized low density lipoprotein
  oxidized low density lipoprotein
  Low density lipoprotein
  MDA oxidized LDL
  MDA oxidized low density lipoprotein.

• 应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:3ug/Test

•  

  

  Fig1: Blank control: A431.
  Primary Antibody (green line): Rabbit Anti-ox-LDL antibody (
  Dilution: 3μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG .
  Secondary Antibody : Goat anti-rabbit IgG-AF647
  Dilution: 3μg /test.
  Protocol
  The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

   

  

  Fig2: Sample:
  Hcclm3(Human) Cell Lysate at 30 ug
  Primary: Anti-ox-LDL  at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 31 kD
  Observed band size: 31 kD

   

  

  Fig3: Paraformaldehyde-fixed, paraffin embedded (Human liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ox-LDL) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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