Anti-OVOL2 antibody

Anti-OVOL2 antibody

• 概述

• 产品描述OVOL2 (Ovo like 2) contains 4 C2H2 type zinc fingers. It belongs to the krueppel C2H2 type zinc finger protein family. It is a DNA binding protein that binds to the 5'-G[GCT]GGGGG-3' core sequence. It probably acts as a transcription regulator.
• 产品名称Anti-OVOL2 antibody
• 分子量30 KDa
• 种属反应性Human,Mouse,Rat,Pig,Cow
• 验证应用WB,IHC-P
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human OVOL2 75-180/275
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Nuclear.
• 其它名称bA504H3.3
  EUROIMAGE566589
  hOvo 2
  hOvo2
  Ovo like 2 (Drosophila)
  Ovo like 2
  OVOL 2
  Transcription factor Ovo like 2
  Zinc finger protein 339
  ZNF 339
  ZNF339
  OVOL2_HUMAN.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Paraformaldehyde-fixed, paraffin embedded (rat liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (OVOL2) Polyclonal Antibody, Unconjugatedat 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

   

  

  Fig2: Sample:
  Testis(Mouse) Cell Lysate at 40 ug
  Primary: Anti-OVOL2 at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 30 kD
  Observed band size: 30 kD

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！