Anti-GTP cyclohydrolase 1 antibody

Anti-GTP cyclohydrolase 1 antibody

• 概述

• 产品描述Positively regulates nitric oxide synthesis in umbilical vein endothelial cells (HUVECs). May be involved in dopamine synthesis. May modify pain sensitivity and persistence. Isoform GCH-1 is the functional enzyme, the potential function of the enzymatically inactive isoforms remains unknown.
• 产品名称Anti-GTP cyclohydrolase 1 antibody
• 分子量27 KDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IHC-P
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human GTP-CH-1 34-110/250
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Cytoplasm. Nucleus.
• 其它名称GTP-CH-1
  DYT 5
  DYT5
  GCH 1
  GCH
  GCH1
  GTP CH 1
  GTP CH I
  GTP cyclohydrolase 1 (dopa responsive dystonia)
  GTP cyclohydrolase 1
  GTP cyclohydrolase I
  GTPCH 1
  GTPCH1
  Guanosine 5' triphosphate cyclohydrolase I
  DYT14
  DYT5a
  HPABH4B.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Sample:Liver (Mouse) Lysate at 40 ug
  Primary: Anti-GTP cyclohydrolase 1 at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 27 kD
  Observed band size: 27 kD

   

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (GTP cyclohydrolase 1) Polyclonal Antibody, Unconjugatedat 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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