Anti-GABRB2 antibody

Anti-GABRB2 antibody

• 概述

• 产品描述GABA, the major inhibitory neurotransmitter in the vertebrate brain, mediates neuronal inhibition by binding to the GABA/benzodiazepine receptor and opening an integral chloride channel.

• 产品名称Anti-GABRB2 antibody

• 分子量56 KDa

• 种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Sheep

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human GABRB2/GABA A Receptor beta 2 251-350/512

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

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• ​其它名称GABA A Receptor beta 2
  GABA
  GABA(A) receptor subunit beta-2
  GABRB2
  Gamma aminobutyric acid (GABA) A receptor, beta 2
  Gamma aminobutyric acid A receptor beta 2
  Gamma-aminobutyric acid receptor subunit beta-2
  Gamma-aminobutyric-acid receptor subunit beta-2
  GBRB2_HUMAN,

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

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  Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (GABRB2) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig2: Sample:
  Cerebellum (rat) Lysate at 40 ug
  Cerebellum (mouse) Lysate at 40 ug
  Primary: Anti- GABRB2 at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 56kD
  Observed band size: 56 kD

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