Anti-GRM3 antibody [G2-G9]

Anti-GRM3 antibody [G2-G9]

• 概述

• 产品描述The mGluR proteins (metabotropic Glutamate Receptors) are members of the G-protein-coupled receptor family and are functionally and pharmacologically distinct from the GluR proteins (ionotropic Glutamate Receptors). The eight currently known mGluR proteins are mediated by two G-proteins with opposing regulation of adenylate cyclase pathways. The activities of mGluR1 and mGluR5 are mediated by a G-protein that activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. The remainder of the eight sub-types of mGluR have an activity mediated by a G-protein that inhibits adenylate cyclase activity. mGluR-3, which may interact with GRASP, acts as a receptor for glutamate.
• 产品名称Anti-GRM3 antibody [G2-G9]
• 分子量99 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2a
• 纯化方式Protein A purified.
• 亚细胞定位Cell membrane
• 其它名称
  • GLUR3 antibody
  • Glutamate metabotropic receptor 3 antibody
  • Glutamate receptor metabotropic 3 antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of GRM3 on human GRM3 recombinant protein using anti-GRM3 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of GRM3 on HEK293 (1) and GRM3-hIgGFc transfected HEK293 (2) cell lysate using anti-GRM3 antibody at 1/1,000 dilution.

   

  

  Fig3: ICC staining GRM3 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-GRM3 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Flow cytometric analysis of SH-SY5Y cells with GRM3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！