Anti-NRF1 antibody [1G1]

Anti-NRF1 antibody [1G1]

• 概述

• 产品描述The NF-E2 DNA binding protein is composed of two subunits, p45 and MafK, and it regulates expression of globin genes in developing erythroid cells through interaction with Maf recognition elements (MAREs). A family of NF-E2 related proteins, which are collectively known as the Cap “n” collar (CNC) family and include Nrf1 (also designated TCF11), Nrf2 and Nrf3, are bZIP transcription factors that heterodimerize with Maf proteins to bind MARE sequences. The Nrf proteins also bind the antioxidant response element (ARE) and are implicated in the regulation of detoxification enzymes and the oxidative stress response. They do so by heterodimerizing with Jun family members (c-Jun, JunB and JunD) to activate gene expression, specifically the detoxifying enzyme, NQO1. Nrf2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from a repressor protein Keap1. Nrf3 is highly expressed in placenta, B cells and monocytes.
• 产品名称Anti-NRF1 antibody [1G1]
• 分子量30kDa
• 种属反应性Human,Mouse
• 验证应用WB,IP,IF,IHC-P
• 抗体类型小鼠单抗
• 免疫原Amino acids 191-475 of Nrf1 of human origin.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃, “DO NOT FREEZE”.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus, Endoplasmic reticulum membrane
• 其它名称
  • alpha pal antibody
  • alpha palindromic binding protein antibody
  • Alpha palindromic-binding protein antibody
  more

• 应用

  WB: 1:100-1:1,000
  IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
  IF: 1:50-1:500
  IHC-P: 1:50-1:500

•  

  

  Fig1: Western blot analysis of Nrf1 expression in non-transfected Hela (A), TF-1 (B), K562 (C), NIH/3T3 (D), RPE-J (E) and 3T3-L1 (F) whole cell lysates.

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded human ovary tissue using anti-Nrf1 antibody. Counter stained with hematoxylin.

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