Anti-CD101 antibody

Anti-CD101 antibody

• 概述

• 产品描述Plays a role as inhibitor of T-cells proliferation induced by CD3. Inhibits expression of IL2RA on activated T-cells and secretion of IL2. Inhibits tyrosine kinases that are required for IL2 production and cellular proliferation. Inhibits phospholipase C-gamma-1/PLCG1 phosphorylation and subsequent CD3-induced changes in intracellular free calcium. Prevents nuclear translocation of nuclear factor of activated T-cell to the nucleus. Plays a role in the inhibition of T-cell proliferation via IL10 secretion by cutaneous dendritic cells. May be a marker of CD4(+) CD56(+) leukemic tumor cells.

• 产品名称Anti-CD101 antibody

• 分子量113 KDa

• 种属反应性Human,Mouse,Rat

• 验证应用WB,ICC/IF,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human CD101 51-150/1021

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Membrane; Single-pass type I membrane protein (Potential).

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• ​其它名称IGSF2
  Immunoglobulin superfamily member 2
  Leukocyte surface protein
  V7
  V7 LSB
  IGSF2_HUMAN
  IgSF2
  Cell surface glycoprotein V7
  Glu-Trp-Ile EWI motif-containing protein 101
  EWI-101
  CD101.

  more

• 应用

  WB:1:500-2000
  FC:1μg/Test
  IF:1:10-100
  ICC/IF:1:100-500

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  Fig1: Protein: HepG2(human) lysates at 30ug;
  Primary: rabbit Anti-CD101at 1:300;
  Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
  Predicted band size:113 kD Observed band size:113 kD

   

  

  Fig2: Blank control(blue): U937(fixed with 2% paraformaldehyde (10 min)).
  Primary Antibody:Rabbit Anti-CD101 antibody, Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
  Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.

• 背景文献

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特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！