Anti-CARD15/NOD2 antibody

Anti-CARD15/NOD2 antibody

• 概述

• 产品名称Anti-CARD15/NOD2 antibody

• 分子量114 KDa

• 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit

• 验证应用WB,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human CARD15 251-350/1040

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Cytoplasm.

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• ​其它名称ACUG
  Arthrocutaneouveal granulomatosis
  BLAU
  CARD15
  Caspase recruitment domain protein 15
  Caspase recruitment domain-containing protein 15
  CD
  IBD1
  Inflammatory bowel disease protein 1
  LRR containing protein
  LRR-containing protein
  Nod2
  NOD2 protein
  NOD2_HUMAN
  NOD2B
  nucleotide binding oligomerization domain 2
  nucleotide-binding oligomerization domain 2
  Nucleotide-binding oligomerization domain-containing protein 2
  PSORAS1.

  more

• 应用

  WB:1:500-2000
  FC:1ug/Test

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  Fig1: Sample:
  MCF-7(Human) Cell Lysate at 30 ug
  Primary: Anti-NOD2 at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 114 kD
  Observed band size: 114 kD

   

  

  Fig2: Sample:
  A549(Human) Cell Lysate at 30 ug
  MCF-7(Human) Cell Lysate at 30 ug
  Primary: Anti-NOD2 at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 114 kD
  Observed band size: 114 kD

   

  

  Fig3: Blank control(blue): Raji(fixed with 2% paraformaldehyde (10 min)).
  Primary Antibody:Rabbit Anti-CARD15/AF488 Conjugated antibody, Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions );

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