Anti-Fibulin 5 antibody
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概述
- 产品描述Fibulin proteins contribute to normal development of elastic fiber systems in various types of organs that require elasticity, such as vasculature, lung and skin. Fibulin-5 (EVEC, UP50, DANCE) is an integrin-binding extracellular matrix protein that mediates endothelial cell adhesion. Fibulin-5 is also a calcium-dependent elastin-binding protein that scaffolds cells to elastic fibers, thereby preventing elastinopathy in the skin, lung, and vasculature. The Arg-Gly-Asp (RGD) motif in Fibulin-5 interacts with cell surface integrins avb3, avb5 and a9b1, serves as an anchorage for elastic fibers to cells, and promotes organization of elastic fibers. The human Fibulin-5 gene maps to chromosome 14q32.12 and encodes a 488 amino acid protein.
- 产品名称Anti-Fibulin 5 antibody
- 分子量50 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Recombinant protein within human Fibulin 5 210-360 aa.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Extracellular matrix. Secreted.
- 其它名称ADCL2 antibody
ARCL1A antibody
ARMD3 antibody
Dance antibody
Developmental arteries and neural crest EGF like protein antibody
Developmental arteries and neural crest EGF-like protein antibody
EVEC antibody
Fbln5 antibody
FBLN5_HUMAN antibody
FIBL 5 antibody
FIBL-5 antibody
Fibulin-5 antibody
FLJ90059 antibody
UP50 antibody
Urine p50 protein antibody
more
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应用
WB: 1:1,000-1:2,000
ICC: 1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
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Fig1: Western blot analysis of Fibulin 5 on human skin tissue lysate using anti-Fibulin 5 antibody at 1/1,000 dilution.
Fig2: ICC staining Fibulin 5 in JAR cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Fibulin 5 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Fibulin 5 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Fibulin 5 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-Fibulin 5 antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of SiHa cells with Fibulin 5 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the s
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