Anti-TAK1 antibody
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概述
- 产品描述Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and Raf-1. Raf-1 is a protein with intrinsic kinase activity towards serine/ threonine residues and that is widely expressed in many tissue types and cell lines. Raf-1 activation is dependent on the small molecular weight GTPase Ras, but the means by which this activation occurs is poorly understood. Two proteins putatively involved in this process are Ksr-1 and Tak1. Ksr-1 (kinase sup-pressor of Ras) is a novel Raf-related protein kinase whose function is required for Ras signal transduction. Whether Ksr-1 lies directly downstream of Ras or acts in a parallel pathway is not yet known. Tak1 (TGFβ-activated kinase) has been shown to participate in the activation of the MAP kinase family in response to TGFβ stimulation.
- 产品名称Anti-TAK1 antibody
- 分子量67 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Synthetic peptide within Human TAK1 aa 560 to the C-terminus .
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified.
- 亚细胞定位Cytoplasm. Membrane.
- 其它名称M3K7_HUMAN antibody
MAP3K 7 antibody
Map3k7 antibody
MEKK7 antibody
Mitogen activated protein kinase kinase kinase 7 antibody
Mitogen-activated protein kinase kinase kinase 7 antibody
TAK1 antibody
TGF beta activated kinase 1 antibody
TGF-beta-activated kinase 1 antibody
TGF1a antibody
Transforming growth factor beta activated kinase 1 antibody
Transforming growth factor-beta-activated kinase 1 antibody
more
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应用
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
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Fig1: ICC staining TAK1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig2: ICC staining TAK1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining TAK1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-TAK1 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-TAK1 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-TAK1 antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of K562 cells with TAK1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary
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