Anti-BHLHB9 antibody

Anti-BHLHB9 antibody

• 概述

• 产品描述This gene is a member of a gene family which encodes proteins with a basic helix-loop-helix domain. Other members of this gene family encode proteins which function as transcription factors, either enhancing or inhibiting transcription depending on the activity of other DNA binding proteins. The coding region of this gene is located entirely within the terminal exon. The encoded protein may be involved in the survival of neurons. Multiple alternatively spliced variants, encoding the same protein, have been identified. May play a role in the control of cellular aging and survival. Highly expressed in brain. Not expressed in lung or liver. Down-regulated in brain from patients suffering from Alzheimer disease.
• 产品名称Anti-BHLHB9 antibody
• 分子量60 kDa
• 种属反应性Human,Mouse
• 验证应用WB,ICC,IHC-P
• 抗体类型兔多抗
• 免疫原Peptide
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG
• 纯化方式Peptide affinity purified
• 亚细胞定位Nucleus. Cytoplasm

• 应用

  WB: 1:500
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200

•  

  

  Fig1: Western blot analysis of BHLHB9 on U937 and SH-SY-5Y cell lysates using anti-BHLHB9 antibody at 1/500 dilution.

   

  

  Fig2: ICC staining BHLHB9 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining BHLHB9 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-BHLHB9 antibody. Counter stained with hematoxylin.

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