Anti-NEDD8 antibody
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概述
- 产品描述NEDD8 is a ubiquitin (Ub)-like molecule that shares 80% homology with ubiquitin, a protein known to modify and target various proteins for proteolytic degradation. NEDD8 and the corresponding yeast homolog Rub1 are activated by the E1 ubiquitin activating enzyme UBA2, which forms isopeptide linkages between thio esters. Similar to the Ub-mediated proteolytic pathway, NEDD8 is covalently coupled to an E3 Ub ligase by the E2 conjugating enzyme, Ubc12. NEDD8 preferentially associates with the CUL-2 protein in the E3 ligase complex CBCVHL, which consists of cullin proteins associating with elongin B/C and the tumor suppressor F-box protein, von Hippel Lindau (VHL). NEDD8 is predominantly localized to the nucleus and is highly expressed in adult heart and skeletal muscle. In vitro studies indicate that NEDD8 tetramers are also able to bind to the 26S Proteasome, and that they assemble into complexes with conserved Ub-like moieties, suggesting that NEDD8 may regulate proteolysis of intracellular proteins similar to other Ub-mediated pathways.
- 产品名称Anti-NEDD8 antibody
- 分子量9 kDa
- 种属反应性Human
- 验证应用WB,ICC,FC
- 抗体类型兔多抗
- 免疫原Recombinant protein
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified
- 亚细胞定位Nucleus.
- 其它名称FLJ43224 antibody
MGC104393 antibody
MGC125896 antibody
MGC125897 antibody
NED8 antibody
NEDD 8 antibody
NEDD-8 antibody
Nedd8 antibody
NEDD8_HUMAN antibody
Neddylin antibody
Neural precursor cell expressed developmentally down regulated 8 antibody
Neural precursor cell expressed developmentally down regulated gene 8 antibody
Neural precursor cell expressed developmentally down-regulated protein 8 antibody
Rub1 antibody
Ubiquitin like protein Nedd 8 antibody
Ubiquitin like protein Nedd8 antibody
Ubiquitin-like protein Nedd8 antibody
more
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应用
WB: 1:500-1:1,000
ICC: 1:50-1:100
FC: 1:50-1:100
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Fig1: Western blot analysis of NEDD8 on PC-3M cell lysate using anti-NEDD8 antibody at 1/1,000 dilution.
Fig2: ICC staining NEDD8 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining NEDD8 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining NEDD8 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Flow cytometric analysis of MCF-7 cells with NEDD8 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
