Anti-Alpha-ENaC antibody
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概述
- 产品描述Sodium permeable non-voltage-sensitive ion channel inhibited by the diuretic amiloride. Mediates the electrodiffusion of the luminal sodium (and water, which follows osmotically) through the apical membrane of epithelial cells. Controls the reabsorption of sodium in kidney, colon, lung and sweat glands. Also plays a role in taste perception.
- 产品名称Anti-Alpha-ENaC antibody
- 分子量76 KDa
- 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse,Sheep
- 验证应用WB,IHC-P,FC
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human Alpha-ENaC 201-300/669
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Apical cell membrane; Multi-pass membrane protein.
- 其它名称Alpha ENaC 2
Alpha ENaC
Alpha NaCH
Alpha-ENaC
Alpha-NaCH
Amiloride sensitive epithelial sodium channel alpha subunit
Amiloride sensitive sodium channel subunit alpha
Amiloride-sensitive sodium channel subunit alpha
ENaCa
ENaCalpha
Epithelial Na(+) channel subunit alpha
Epithelial Na+ channel subunit alpha
FLJ21883
Nonvoltage gated sodium channel 1 subunit alpha
Nonvoltage-gated sodium channel 1 subunit alpha
SCNEA
SCNN 1
SCNN1
SCNN1A
SCNNA_HUMAN
Sodium channel nonvoltage gated 1 alpha.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
FC:5ug/Test
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Fig1: Sample:
Kidney (Mouse) Lysate at 40 ug
Primary: Anti-Alpha-ENaC at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 76 kD
Observed band size: 77 kD
Fig2: Sample:
Kidney (Rat) Lysate at 40 ug
Primary: Anti-Alpha-ENaC at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 76 kD
Observed band size: 77 kD
Fig3: Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Alpha-ENaC Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Fig4: Blank control: A549(blue), the cells were fixed with 2% paraformaldehyde (10 min) and .
Isotype Control Antibody: Rabbit IgG(orange) ; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 5μg in 100 μL1X PBS containing 0.5% BSA(green).
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
