Anti-ADORA2B antibody

Anti-ADORA2B antibody

• 概述

• 产品描述The adenosine A2B receptor, also known as ADORA2B, is a G-protein coupled adenosine receptor, and also denotes the human adenosine A2b receptor gene which encodes it. This integral membrane protein stimulates adenylate cyclase activity in the presence of adenosine. This protein also interacts with netrin-1, which is involved in axon elongation.Research into selective A2B ligands has lagged somewhat behind the development of ligands for the other three adenosine receptor subtypes, but a number of A2B-selective compounds have now been developed, and research into their potential therapeutic applications is ongoing.
• 产品名称Anti-ADORA2B antibody
• 分子量37 KDa
• 种属反应性Human,Mouse,Rat,Dog,Rabbit,Guinea Pig
• 验证应用WB,IHC-P,ICC/IF,FC
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human ADORA2B 101-200/332
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式Affinity purified by Protein A
• 亚细胞定位Cell membrane; Multi-pass membrane protein.
• 其它名称Adenosine A2b Receptor
  adora 2
  adora 2b
  ADORA2
  ADORA2B
  HGNC:264
  Netrin 1 receptor
  AA2BR_HUMAN.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:3ug/test
  IF:1:100-500
  ICC/IF:1:100-500

•  

  

  Fig1: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ADORA2B) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

   

  

  Fig2: Sample: Brain (Mouse) Lysate at 40 ug
  Primary: Anti-ADORA2Bat 1/300 dilution
  Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
  Predicted band size: 37 kD
  Observed band size: 36 kD

   

  

  Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ADORA2B) Polyclonal Antibody, Unconjugatedat 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

   

  

  Fig4: Blank control: A549.
  Primary Antibody (green line): Rabbit Anti-ADORA2B antibody 
  Dilution: 3μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG .
  Secondary Antibody : Goat anti-rabbit IgG-PE
  Dilution: 1μg /test.
  Protocol
  The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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