Anti-ADAM12 antibody

Anti-ADAM12 antibody

• 概述

• 产品描述Involved in skeletal muscle regeneration, specifically at the onset of cell fusion. Also involved in macrophage-derived giant cells (MGC) and osteoclast formation from mononuclear precursors (By similarity).
• 产品名称Anti-ADAM12 antibody
• 分子量77/100 KDa
• 种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Rabbit
• 验证应用WB,FC
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human ADAM12 201-300/909
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted. Isoform 3: Secreted (Potential). Isoform 4: Secreted (Potential).
• 其它名称A disintegrin and metalloproteinase domain 12
  A disintegrin and metalloproteinase domain 12
  ADA12_HUMAN
  ADAM 12
  ADAM metallopeptidase domain 12
  Disintegrin and metalloproteinase domain-containing protein 12
  MCMP
  MCMPMltna
  MCMPMltna
  Meltrin alpha
  Meltrin-alpha
  MLTN
  MLTNA
  OTTHUMP00000046766.
  more

• 应用

  WB:1:500-2000
  FC:3μg/Test

•  

  

  Fig1: Blank control (Black line): U87MG (Black).
  Primary Antibody (green line): Rabbit Anti-ADAM12 antibody
  Dilution: 1μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG.
  Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
  Dilution: 1μg /test.
  Protocol
  The cells were fixed with 4% PFA (10min at room temperature)and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

   

  

  Fig2: Sample:
  Heart (Mouse) Lysate at 40 ug
  Primary: Anti-ADAM12at 1/500 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 77/100 kD
  Observed band size: 115 kD

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！