Anti-5HT2B Receptor antibody
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概述
- 产品描述This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. Plays a role in the regulation of impulsive behavior.
- 产品名称Anti-5HT2B Receptor antibody
- 分子量54 KDa
- 种属反应性Human
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human 5HT2B Receptor/SR-2B 1-100/481
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Cell membrane; Multi-pass membrane protein.
- 其它名称SR-2B
5 HT 2B
5 HT 2B receptor
5 HT(2B)
5 HT2B
5 hydroxytryptamine (serotonin) receptor 2B
5 hydroxytryptamine (serotonin) receptor 2B G protein coupled
5 hydroxytryptamine 2B receptor
5 hydroxytryptamine receptor 2B
5-HT-2B
5-HT2B
5-hydroxytryptamine receptor 2B
5HT(2B)
5HT2B_HUMAN
5HT2F
Htr 2b
Htr2b
NP75 protein
SEROTONIN 5HT2B RECEPTOR
Serotonin receptor 2B
Stomach fundus serotonin receptor.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Sample:
MCF-7 Cell (Human) Lysate at 30 ug
Primary: Anti- 5HT2A Receptorat 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 54kD
Observed band size: 54/59kD
Fig2: Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (5HT2B Receptor) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
