Anti-ASK1 antibody
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概述
- 产品描述Mitogen-activated protein (MAP) kinase cascades are activated by various extracellular stimuli including growth factors. The MEK kinases (also designated MAP kinase kinase kinases, MKKKs, MAP3Ks or MEKKs) phosphorylate and thereby activate the MEKs (also called MAP kinase kinases or MKKs), including ERK, JNK and p38. These activated MEKs in turn phosphorylate and activate the MAP kinases. The MEK kinases include Raf-1, Raf-B, Mos, MEK kinase-1, MEK kinase-2, MEK kinase-3, MEK kinase-4, ASK 1 (MEK kinase-5) and MAP3K6 (MEK kinase-6). MEK kinase-1 has been shown to phosphorylate MEK-1 via a Raf-independent pathway. Evidence suggests that MEK-3 is preferentially activated by MEK kinase-3 and that MEK-4 is activated by both MEK kinase-2 and MEK kinase-3. MEK kinase-4 has been shown to specifically activate the JNK pathway. ASK1 activates both MEK-4 and MEK-3/MEK-6 pathways.
- 产品名称Anti-ASK1 antibody
- 分子量155 kDa
- 种属反应性Human,Mouse
- 验证应用WB,ICC,IHC-P
- 抗体类型兔多抗
- 免疫原Synthetic peptide of C-terminal human ASK1.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified.
- 亚细胞定位Cytoplasm. Endoplasmic reticulum.
- 其它名称Apoptosis signal regulating kinase 1 antibody
Apoptosis signal-regulating kinase 1 antibody
ASK 1 antibody
ASK-1 antibody
ASK1 antibody
M3K5 antibody
M3K5_HUMAN antibody
MAP/ERK kinase kinase 5 antibody
MAP3K5 antibody
MAPK/ERK kinase kinase 5 antibody
MAPKKK5 antibody
MEK kinase 5 antibody
MEKK 5 antibody
MEKK5 antibody
Mitogen activated protein kinase kinase kinase 5 antibody
Mitogen-activated protein kinase kinase kinase 5 antibody
more
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应用
WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
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Fig1: Western blot analysis of ASK1 on human skeletal muscle tissue lysate using anti-ASK1 antibody at 1/500 dilution.
Fig2: ICC staining ASK1 (green) in PANC-1 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-ASK1 antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ASK1 antibody. Counter stained with hematoxylin.
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