Anti-5HT3B receptor antibody
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概述
- 产品描述This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses. It is a cation-specific, but otherwise relatively nonselective, ion channel.
- 产品名称Anti-5HT3B receptor antibody
- 分子量50 KDa
- 种属反应性Human,Mouse,Rat,Cow,Rabbit
- 验证应用WB,IHC-P,FC
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human 5HTR3B/5HT3B receptor 101-200/441
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Cell membrane; Multi-pass membrane protein. Note=Presumably retained within the endoplasmic reticulum unless complexed with HTR3A.
- 其它名称5 HT3 B
5 HT3B
5 HTR3B
5 hydroxytryptamine 3 receptor B subunit
5 hydroxytryptamine receptor 3B
5 hydroxytryptamine serotonin receptor 3B
5-HT3-B
5-HT3B
5-HTR3B
5-hydroxytryptamine 3 receptor B subunit
5-hydroxytryptamine receptor 3B
5-hydroxytryptamine serotonin receptor 3B
5HT3B_HUMAN
5HTR3B
HTR3B
Serotonin gated ion channel subunit
serotonin receptor 3B.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
FC:1ug/Test
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Fig1: Blank control(blue):293T Cells(fixed with 2% paraformaldehyde (10 min) and then permeabilized with ice-cold 90% methanol for 30 min on ice).
Primary Antibody: Rabbit Anti-5HT3B receptor Phospho-c-Fos (Thr325)/AF488 Conjugated antibodyDilution: 1ug in 100 ul 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF488(orange) ,used under the same conditions.
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (5HT3B receptor) Polyclonal Antibody, Unconjugatedat 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig3: Sample: 293T Cell (Human) Lysate at 40 ug
Primary: Anti- 5HT3B receptor at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 55 kD
Fig4: Sample: Lovo Cell (Human) Lysate at 40 ug
Primary: Anti- 5HT3B receptor at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 55 kD
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