Anti-CYP11B1 antibody

Anti-CYP11B1 antibody

• 概述

• 产品描述Has steroid 11-beta-hydroxylase activity. In addition to this activity, the 18 or 19-hydroxylation of steroids and the aromatization of androstendione to estrone have also been ascribed to cytochrome P450 XIB.
• 产品名称Anti-CYP11B1 antibody
• 分子量55 KDa
• 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep
• 验证应用WB,IHC-P,ICC/IF
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human CYP11B1 301-400/503
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
  
• 其它名称CPN1
  CYP11B
  CYPXIB1
  Cytochrome P450 11B1, mitochondrial
  Cytochrome p450 XIB1
  Cytochrome P450, family 11, subfamily B, polypeptide 1
  FHI
  P 450C11
  P450C11
  S11BH
  Steroid 11 beta hydroxylase
  Steroid 11 beta monooxygenase
  C11B1_HUMAN
  Cytochrome P-450c11
  Cytochrome P450C11
  Steroid 11-beta-hydroxylase.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800
  IF:1:100-500

•  

  

  Fig1: Sample:
  Kidney (Rat) Lysate at 40 ug
  Brain (Rat) Lysate at 40 ug
  Primary: Anti-CYP11B1 at 1/300 dilution
  Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
  Predicted band size: 55 kD
  Observed band size: 55 kD

   

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation withPolyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

   

  

  Fig3: Sample:
  Heart (Mouse) Lysate at 40 ug
  Primary: Anti- CYP11B1at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 55 kD
  Observed band size: 55 kD

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