Anti-CXCL16 antibody
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概述
- 产品描述Chemokine (C-X-C motif) ligand 16 (CXCL16) is a small cytokine belonging to the CXC chemokine family. Larger than other chemokines (with 254 amino acids), CXCL16 is composed of a CXC chemokine domain, a mucin-like stalk, a transmembrane domain and a cytoplasmic tail containing a potential tyrosine phosphorylation site that may bind SH2. These are unusual features for a chemokine, and allow CXCL16 to be expressed as a cell surface bound molecule, as well as a soluble chemokine. CXCL16 is produced by dendritic cells found in the T cell zones of lymphoid organs, and by cells found in the red pulp of the spleen. Cells that bind and migrate in response to CXCL16 include several subsets of T cells, and natural killer T (NKT) cells. CXCL16 interacts with the chemokine receptor CXCR6, also known as Bonzo. Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha. The gene for human CXCL16 is located on chromosome 17.
- 产品名称Anti-CXCL16 antibody
- 分子量27 KDa
- 种属反应性Human, Mouse, Rat
- 验证应用WB, IHC-P, ICC/IF, FC
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from the middle of human CXCL16 85-200/247
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式Affinity purified by Protein A.
- 亚细胞定位Cell membrane. Secreted. Also exists as a soluble form.
- 其它名称CXC-chemokine ligand 16
Chemokine (C X C motif) ligand 16
CXC chemokine ligand 16
CXCLG16
Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein
SCYB16
Small inducible cytokine B16 precursor
SRPSOX
Transmembrane chemokine CXCL16
CXL16_HUMAN.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
FC:3ug/Test
IF:1:100-500
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Fig1: Sample: BV2 Cell (Mouse) Lysate at 40 ug
Primary: Anti- CXCL16 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 27 kD
Observed band size: 27 kD
Fig2: Blank control: A431.
Primary Antibody (green line): Rabbit Anti-CXCL16 antibody
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Fig3: Images kindly provided by Dr. Denny Cottle from the publication, Fetal inhibition of inflammation improves disease phenotypes in Harlequin Ichthyosis.Formalin-fixed and paraffin embedded mouse skin probed with Rabbit Anti-CXCL16 Polyclonal Antibody at 1:50 for two hours at room temperature (green channel).
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
