Anti-FE65 antibody

Anti-FE65 antibody

• 概述

• 产品描述Transcription coregulator that can have both coactivator and corepressor functions. Adapter protein that forms a transcriptionally active complex with the gamma-secretase-derived amyloid precursor protein (APP) intracellular domain. Plays a central role in the response to DNA damage by translocating to the nucleus and inducing apoptosis. May act by specifically recognizing and binding histone H2AX phosphorylated on 'Tyr-142' (H2AXY142ph) at double-strand breaks (DSBs), recruiting other pro-apoptosis factors such as MAPK8/JNK1. Required for histone H4 acetylation at double-strand breaks (DSBs). Its ability to specifically bind modified histones and chromatin modifying enzymes such as KAT5/TIP60, probably explains its trancription activation activity. Function in association with TSHZ3, SET and HDAC factors as a transcriptional repressor, that inhibits the expression of CASP4. Associates with chromatin in a region surrounding the CASP4 transcriptional start site(s).
• 产品名称Anti-FE65 antibody
• 分子量77 KDa
• 种属反应性Human,Mouse,Rat,Rabbit
• 验证应用WB,IHC-P
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human Fe65 protein 201-300/710
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Cell membrane. Cytoplasm. Nucleus. Cell projection, growth cone. Nucleus speckle. Note=Colocalizes with TSHZ3 in axonal growth cone. In normal conditions, it mainly localizes to the cytoplasm, while a small fraction is tethered to the cell membrane via it
• 其它名称Adaptor protein FE65a2
  Amyloid beta (A4) precursor protein binding family B member 1
  Amyloid beta A4 precursor protein binding family B
  Amyloid beta A4 precursor protein binding family B member 1
  Amyloid beta precursor protein binding family B member 1
  APBB 1
  APBB1
  FE 65
  Fe65 protein
  MGC 9072
  MGC9072
  Protein Fe65
  RIR
  Stat like protein
  Fe-65 Protein
  APBB1_HUMAN.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Sample: Cerebrum (Mouse) Lysate at 40 ug
  Primary: Anti- FE65  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 77 kD
  Observed band size: 75 kD

   

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (FE65) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (FE65) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig4: Sample: Cerebrum (Rat) Lysate at 40 ug
  Primary: Anti- FE65 at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 77 kD
  Observed band size: 75 kD

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！