Anti-Alpha-tubulin antibody

Anti-Alpha-tubulin antibody

• 概述

• 产品描述The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
• 产品名称Anti-Alpha-tubulin antibody
• 分子量50 kDa
• 种属反应性Human, Mouse, Rat, Zebrafish
• 验证应用WB, IHC-P, ICC, FC, IF
• 抗体类型兔多抗
• 免疫原peptide
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG
• 纯化方式Peptide affinity purified
• 亚细胞定位Cytoplasm ,cytoskeleton
• 其它名称TUBA1 antibody
  TUBA1A antibody
  TUBA2 antibody
  TUBA3 antibody
  TUBA3C antibody
  TUBA4A antibody
  Tubulin, alpha 1a antibody
  Tubulin, alpha 3c antibody
  Tubulin, alpha 4a antibody
  more

• 应用

  WB: 1:5,000-1:10,000
  ICC: 1:200-1:500
  IHC-P: 1:200-1:500
  IF: 1:200-1:500
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of Alpha-tubulin on different cell lysates using anti-Alpha -tubulin antibody at 1/10000 dilution.
  Positive control:
  Lane 1: NIH/3T3
  Lane 2: HepG2
  Lane 3: PC12
  Lane 4: Hela

   

  

  Fig2: ICC staining of Alpha-tubulin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining of Alpha-tubulin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: IHC-P image of Alpha-tubulin staining with Alpha-tubulin antibody on tissue sections from human stomach. The secondary used was an Alexa-Fluor 555 conjugated goat anti-rabbit polyclonal.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Alpha-tubulin antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Alpha-tubulin antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of Hela cells with Alpha-tubulin antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.

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