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别名: | EPOR | ||
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适用物种: | Human,Mouse,Rat,Dog,Cow,Horse | ||
验证应用: | WB,IHC-P,FC | ||
种属: | 兔多抗 | ||
储存条件: | -20℃ |
货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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熔点: | |
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密度: | |
储存条件: | -20℃ |
Anti-EPOR antibody
WB:1:500-2000
IHC-P:1:400-800
FC:1µg/Test
Fig1: Blank control: Molt-4 Cells(blue).
Primary Antibody: Rabbit Anti-EPOR/FITC Conjugated antibody Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF488 orange) ,used under the same conditions.
Protocol
The cells were fixed with 2% paraformaldehyde (10 min) . The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (ER1908-28/AF488, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.
Fig2: Sample:
K562(Human) Cell Lysate at 30 ug
Primary: Anti-EPORat 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 30/52 kD
Fig3: Sample:
K562(Human) Cell Lysate at 30 ug
Primary: Anti- EPORat 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 56 kD
Observed band size: 64 kD
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