Anti-EphB2 antibody

Anti-EphB2 antibody

• 概述

• 产品名称Anti-EphB2 antibody
• 分子量114 KDa
• 种属反应性Human,Mouse,Rat,Chicken,Dog
• 验证应用WB,IHC-P
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human EphB2 R 101-200/1055
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Cell membrane; Single-pass type I membrane protein. Cell projection, axon. Cell projection, dendrite.
• 其它名称Eph receptor B2
  Developmentally regulated EPH related tyrosine kinase
  DRT
  ELK related protein tyrosine kinase
  EPH tyrosine kinase 3
  EphB2
  Ephrin type B receptor 2
  EPHT 3
  ERK
  ETECK
  Nuk
  Prkm 5
  Receptor protein tyrosine kinase HEK 5, Sek 3
  Tyro 5
  Tyrosine protein kinase receptor CEK 5
  Tyrosine protein kinase receptor EPH 3
  Tyrosine protein kinase receptor QEK 5
  EPHB2_HUMAN.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Sample:
  Testis (Mouse) Lysate at 40 ug
  Primary: Anti-EphB2 R at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 114 kD
  Observed band size: 114 kD

   

  

  Fig2: Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-EphB2 R Polyclonal Antibody, Unconjugated1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

   

  

  Fig3: Tissue/cell: human colon carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-EphB2 R Polyclonal Antibody, Unconjugate1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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