Anti-Wnt11 antibody

Anti-Wnt11 antibody

• 概述

• 产品描述Wnt11 is a non-canonical Wnt which is expressed in androgen-independent prostate cancer cell lines and prostate tumours. It is involved in development, including cardiac and gut development.
• 产品名称Anti-Wnt11 antibody
• 分子量36 KDa
• 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit
• 验证应用WB,IHC-P
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human Wnt11 151-250/354
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Secreted; extracellular space; extracellular matrix.
• 其它名称HWNT11
  Protein Wnt-11
  Wingless-related MMTV integration site 11
  Wingless-type MMTV integration site family, member 1
  Wingless-type MMTV integration site family, member 11
  Wingless-type MMTV integration site family, member 11B
  wnt-11
  wnt11b.WNT11_HUMAN
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Sample:
  Heart (Mouse) Lysate at 40 ug
  Primary: Anti- Wnt11 at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 36 kD
  Observed band size: 34 kD

   

  

  Fig2: Sample:
  Embryo (Mouse) Lysate at 40 ug
  293T(Human) Cell Lysate at 30 ug
  Primary: Anti- Wnt11at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 36 kD
  Observed band size: 35 kD

   

  

  Fig3: Paraformaldehyde-fixed, paraffin embedded (mouse liver tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Wnt11) Polyclonal Antibody, Unconjugatedat 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig4: Sample:
  Liver (Mouse) Lysate at 40 ug
  Primary: Anti- Wnt11 at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 36 kD
  Observed band size: 63 kD

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！