Anti-BASP1 antibody

Anti-BASP1 antibody

• 概述

• 产品描述BASP1 is a brain abundant membrane bound protein with several transient phosphorylation sites and PEST motifs. BASP1 binds calmodulin, is phosphorylated by protein kinase C and undergoes N-terminal myristoylation. BASP1 is expressed specifically in nervous tissue. Intercellular signaling and nerve outgrowth are influenced by a number of proteins expressed at nerve endings and synapses of which BASP1 is one.
• 产品名称Anti-BASP1 antibody
• 分子量25 KDa
• 种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit
• 验证应用WB,IHC-P
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human BASP1/Nap22 3-100/227
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Cell membrane; Lipid-anchor. Cell projection, growth cone. Note=Associated with the membranes of growth cones that form the tips of elongating axons.
• 其它名称BASP 1
  BASP-1
  BASP1 protein
  Brain abundant membrane attached signal protein 1
  Brain Abundant Signal Protein Membrane Attached 1
  Brain Acid Soluble Protein 1
  CAP 23
  CAP23
  MGC8555
  NAP 22
  NAP-22
  NAP22
  Neuronal axonal membrane protein NAP 22
  Neuronal tissue enriched acidic protein
  22 kDa neuronal tissue enriched acidic protein
  BASP1_HUMAN.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (BASP1) Polyclonal Antibody, Unconjugatedat 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig2: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-BASP1 Polyclonal Antibody, Unconjugated(ER1904-13) 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

   

  

  Fig3: Sample:Hela (Human)Cell Lysate at 40 ug
  Primary: Anti-BASP1at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
  Predicted band size: 25kD
  Observed band size: 26kD

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