Anti-PP2C alpha antibody
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概述
- 产品描述The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/ threonine protein phosphatases. Protein phosphatase 2Ca (PP2Ca) has broad specificity. It dephosphorylates and negatively regulates the activities of MAP kinases and MAP kinase-kinases while also inhibiting the activation of p38 and JNK kinase cascades, induced by environmental stresses. PP2Ca also induces the expression of endogenous p53 and the p53-responsive gene p21, leading to cell cycle arrest and apoptosis. The PP2Ca protein, which contains an active site containing a dinuclear metal ion center, shows highest expression in epithelial cells, as well as in the digestive tract, lung, kidney, breast, prostate, endocrine glands and brain.
- 产品名称Anti-PP2C alpha antibody
- 分子量42/35 kDa, additional band 55 kDa.
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Recombinant protein
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein A purified.
- 亚细胞定位Cytoplasm, Membrane, Nucleus.
- 其它名称Protein phosphatase 2C isoform alpha antibody
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FLJ42306 antibody IA antibody
MGC9201 antibody
Mpp alpha antibody
PP2C alpha antibody
PP2C-alpha antibody
PP2CA antibody
PP2Calpha antibody
PPM 1A antibody
PPM1A antibody
PPM1A_HUMAN antibody
PPPM1A antibody
Protein phosphatase 1A (formerly 2C) magnesium dependent alpha isoform antibody
Protein phosphatase 1A antibody
Protein phosphatase 1A magnesium dependent alpha antibody
Protein phosphatase 2C alpha antibody
Protein phosphatase 2C alpha isoform antibody
Protein phosphatase 2C isoform alpha antibody
Protein phosphatase IA antibody
Protein phosphatase, Mg2+/Mn2+ dependent, 1A antibody
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应用
WB: 1:500
ICC: 1:100-1:500
IHC-P: 1:50-1:200
FC: 1:50-1:100
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Fig1: Western blot analysis of PP2C alpha on mouse kidney tissue (1) and rat skin tissue (2) lysate using anti-PP2C alpha antibody at 1/100 dilution.
Fig2: ICC staining PP2C alpha in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining PP2C alpha in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining PP2C alpha in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PP2C alpha antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PP2C alpha antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-PP2C alpha antibody. Counter stained with hematoxylin.
Fig8: Flow cytometric analysis of A431 cells with PP2C alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the seco
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