Anti-ROBO1 antibody
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概述
- 产品描述Specific cells in the midline separate the left and right halves of the central nervous system and play many roles in guiding the growth cone behavior. In vertebrate spinal cord, insect abdominal nerves and nematodes, midline cells produce induced cues, such as nectins and slits, respectively, as attractants and repellents. respectively. These cells can serve as gatekeepers to prevent axons from passing through the midline and to guide the growth cone in response to the switching of lead cues beyond the crossing. One such gatherer, Robo, is an axon guidance receptor that defines a new subfamily of proteins from fruit flies to mammalian conserved Ig superfamily proteins. Robo acts as a receptor for the repellent Slit and functions in a cell-autonomous manner Non-intersecting axons express high levels of Robo and cross axons to express low levels of Robo and then reach midline and high levels of crossover. Robo1 and Robo2 are two human homologs of Drosophila megalopolis. Robo1 is also homologous to the C. elegans gene sax3, while Robo2 is homologous to zebrafish genes.
- 产品名称Anti-ROBO1 antibody
- 分子量180 kDa
- 种属反应性Human,Mouse
- 验证应用ICC,IHC-P
- 抗体类型兔多抗
- 免疫原Peptide
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified
- 亚细胞定位Membrane.
- 其它名称Deleted in U twenty twenty antibody
DUTT 1 antibody
DUTT1 antibody
FLJ21882 antibody
H Robo 1 antibody
H-Robo-1 antibody
hRobo 1 antibody
Robo 1 antibody
Robo1 antibody
ROBO1_HUMAN antibody
Roundabout 1 antibody
Roundabout axon guidance receptor homolog 1 antibody
Roundabout homolog 1 antibody
Roundabout homolog1 precurser antibody
Roundabout1 antibody
SAX 3 antibody
SAX3 antibody
more
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应用
ICC: 1:100-1:500
IHC-P: 1:50-1:200
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Fig1: ICC staining ROBO1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig2: ICC staining ROBO1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining ROBO1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ROBO1 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-ROBO1 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ROBO1 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ROBO1 antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ROBO1 antibody. Counter stained with hematoxylin.
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