Anti-Nociceptin receptor antibody
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概述
- 产品描述Receptor for the neuropeptide nociceptin/orphanin FQ. Has a potential role in modulating a number of brain functions, including instinctive behaviors and emotions. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase.
- 产品名称Anti-Nociceptin receptor antibody
- 分子量40 KDa
- 种属反应性Human,Mouse,Rat,Dog,Pig,Cow,Horse,Guinea Pig
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human Nociceptin receptor 1-47/370
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Cell membrane; Multi-pass membrane protein.
- 其它名称H.sapiens mRNA for ORL1 receptor
Kappa type 3 opioid receptor
Kappa-type 3 opioid receptor
KOR 3
KOR-3
KOR3
LC132 receptor like
MGC34578
Nociceptin receptor
NOCIR
OOR
Opiate receptor like 1
Opiod receptor like 1
Oprl1
OPRX_HUMAN
ORL1
Orphanin FQ receptor.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti- Nociceptin receptorat 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 52 kD
Fig2: Sample:
Heart(Rat) lysate at 30ug;
Brain(Rat) lysate at 30ug;
Primary: Anti-Nociceptin receptor (at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000 dilution;
Predicted band size : 40kD
Observed band size : 40kD
Fig3: Immunofluorescence.
Tissue/cell: human placenta tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Nociceptin receptor Polyclonal Antibody, Unconjugated1:200, overnight at 4℃; The secondary antibody was Goat Anti-Rabbit IgG, Cy3conjugated (bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37℃.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
