Anti-BCAT1 antibody
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概述
- 产品描述Branched chain amino acid transaminase 1 is a protein that in humans is encoded by the BCAT1 gene. It is the first enzyme in the Branched-chain amino acid (BCAA) degradation pathway and facilitates the reversible transamination of BCAAs and glutamate. BCAT1 resides in the cytoplasm, while its isoform, BCAT2, is found in the mitochondria. This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids (BCKAs) to the branched-chain amino acids (BCAAs) Valine, Leucine and Isoleucine, which are essential for cell growth. In humans, its primary role is the deamination of BCAAs, as humans lack the enzymes for de novo synthesis of BCKAs. The respective cofactors are alpha-ketoglutarate and glutamate. Cells can further degrade BCKAs by the Branched-chain keto acid dehydrogenase complex from which the carbon backbones of each BCAA may enter distinct degradation pathways. The oncogenic transcription factor Myc is frequently reported to drive BCAT1 expression.
- 产品名称Anti-BCAT1 antibody
- 分子量43 KDa
- 种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Sheep
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human BCAT1 321-386/386
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式Affinity purified by Protein A
- 亚细胞定位Cytoplasm.
- 其它名称cytosolic
BCAT
BCAT(c)
Bcat1
BCAT1_HUMAN
BCT1
Branched chain amino acid aminotransferase, cytosolic
Branched-chain-amino-acid aminotransferase
ECA39
MECA39
PNAS 121
Protein ECA39.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-BCAT1at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 45 kD
Fig2: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-BCAT1 Polyclonal Antibody, Unconjugated1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Fig3: Sample: Brain (Mouse) Lysate at 40 ug
Primary: Anti-BCAT1 at 1/300 dilution
Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
Predicted band size: 43 kD
Observed band size: 43 kD
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