Anti-CD79a antibody [C1-E8]

Anti-CD79a antibody [C1-E8]

• 概述

• 产品描述Required in cooperation with CD79B for initiation of the signal transduction cascade activated by binding of antigen to the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. Also required for BCR surface expression and for efficient differentiation of pro- and pre-B-cells. Stimulates SYK autophosphorylation and activation. Binds to BLNK, bringing BLNK into proximity with SYK and allowing SYK to phosphorylate BLNK. Also interacts with and increases activity of some Src-family tyrosine kinases. Represses BCR signaling during development of immature B-cells.
• 产品名称Anti-CD79a antibody [C1-E8]
• 分子量Predicted band size 25 kDa.
• 种属反应性Human,Mouse
• 验证应用WB,FC
• 抗体类型小鼠单抗
• 免疫原Synthetic peptide within mouse CD79a aa 100-200.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/ml.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG
• 纯化方式Protein A purified.
• 亚细胞定位Cell membrane, Membrane
• 其它名称
  • B lymphocyte-specific MB1 protein antibody
  • B-cell antigen receptor complex-associated protein alpha chain antibody
  • CD 79a antibody
  more

• 应用

  WB: 1:500-1:1,000
  FC: 1:50-1:100

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  Fig1: Western blot analysis of CD79a on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
  Positive control:
  Lane 1: Human thymus tissue lysate
  Lane 2: Mouse spleen tissue lysate
  Lane 3: Daudi cell lysate

   

  

  Fig2: Flow cytometric analysis of CD79a was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-mouse IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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