Anti-SDHB antibody
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概述
- 产品描述In aerobic respiration reactions, succinate dehydrogenase (SDH) catalyzes the oxidation of succinate and ubiquinone to fumarate and ubiquinol. Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB) and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. Mutations to these subunits cause mitochondrial dysfunction, corresponding to several distinct disorders. Mutations in the membrane bound components may cause hereditary paraganglioma, while SDHA mutations associate with juvenile encephalopathy as well as Leigh syndrome, a severe neurological disorder. Inactivating mutations in SDHB correlate with inherited, but not necessarily sporadic, cases of pheochromocytoma.
- 产品名称Anti-SDHB antibody
- 分子量32 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Recombinant protein within human SDHB aa 100-300.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Mitochondrion.
- 其它名称CWS2 antibody
DHSB_HUMAN antibody
FLJ92337 antibody
Ip antibody
Iron sulfur subunit antibody
Iron sulfur subunit of complex II antibody
Iron-sulfur subunit of complex II antibody
mitochondrial antibody
PGL 4 antibody
PGL4 antibody
SDH 1 antibody
SDH antibody
SDH1 antibody
SDH2 antibody
SDH2, homolog of antibody
SdhB antibody
SDHIP antibody
Succinate dehydrogenase [ubiquinone] iron sulfur protein mitochondrial antibody
Succinate dehydrogenase [ubiquinone] iron sulfur subunit antibody
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit antibody
succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial antibody
Succinate Dehydrogenase 1 Iron Sulfur Subunit antibody
Succinate Dehydrogenase 2, S. cerevisiae, homolog of antibody
Succinate dehydrogenase complex iron sulfur subunit B antibody
Succinate dehydrogenase complex subunit B iron sulfur antibody
Succinate Dehydrogenase Complex Subunit B Iron Sulfur Protein antibody
succinate dehydrogenase complex, subunit B, iron sulfur (Ip) antibody
Succinate dehydrogenase iron sulfur protein antibody
more
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应用
WB: 1:500-1:2,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
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Fig1: Western blot analysis of SDHB on different cell lysate using anti-SDHB antibody at 1/1,000 dilution.
Positive control:
Lane 1: human liver
Lane 2: mouse liver
Lane 3: rat liver
Lane 4: rat liver, preincubated with the immunization protein.
Fig2: ICC staining SDHB in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining SDHB in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-SDHB antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SDHB antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-SDHB antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SDHB antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-SDHB antibody. Counter stained with hematoxylin.
Fig9: Flow cytometric analysis of 293T cells with SDHB antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary
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