Anti-GTSF1 antibody
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概述
- 产品描述Gametocyte-specific factor 1 (Gtsf1), also known as the computationally obtained undifferentiated and/or embryonic stem cell-specific 110 gene (Cue110). Gtsf1 encodes a 167-amino acid protein that is a member of the Uncharacterized Protein Family 0224. DmGTSF1 is essential for P-element-induced wimpy testis (PIWI)-interacting RNA (piRNA)-mediated transcriptional repression and histone H3K9me3-mediated repression of transposons and their neighboring genes in the ovary. DmGTSF1 interacts with Piwi via its C-terminal tail and the two CHHC zinc-finger motifs within the DmGTSF1 protein are required for its activity. The Gtsf1 gene is essential for spermatogenesis and has been revealed to function in transposon suppression in the mouse testes. GTSF1 transcript expression has been observed to be up-regulated in certain leukemias and in lymphomas.
- 产品名称Anti-GTSF1 antibody
- 分子量35 kDa, predicted band size 21 kDa
- 种属反应性Zebrafish
- 验证应用WB,IF,IHC-P
- 抗体类型兔多抗
- 免疫原Recombinant protein within zebrafish GTSF1 aa 20-170.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/ml.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Cytoplasm.
- 其它名称Cue110 antibody
Fam112b antibody
Family with sequence similarity 112 member B antibody
FLJ32942 antibody
Gametocyte-specific factor 1 antibody
GTSF1 antibody
GTSF1_HUMAN antibody
Protein FAM112B antibody
more
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应用
WB:1:200
IF:1:50-1:200
IHC-P:1:50-1:200
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Fig1: Western blot analysis of GTSF1 on zebrafish lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:200 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: Immunofluorescence staining of paraffin-embedded Zebrafish using anti-GTSF1 rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the antibody (ER1803-68) at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor™ 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.
Fig3: Immunohistochemical analysis of paraffin-embedded Zebrafish tissue using anti-GTSF1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1803-68) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
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