Anti-CASK antibody
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概述
- 产品描述Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TBR1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1.
- 产品名称Anti-CASK antibody
- 分子量105/59 kDa
- 种属反应性Human,Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Recombinant protein within human CASK aa 300-500.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Plasma membrane. Nucleus.
- 其它名称CAGH39 antibody
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应用
WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC-P: 1:100-1:200
FC: 1:50-1:100
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Fig1: Western blot analysis of CASK on different cell lysate using anti-CASK antibody at 1/500 dilution.
Positive control:
Lane 1: SiHa
Lane 2: A549
Fig2: ICC staining CASK in HT-29 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining CASK in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CASK antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-CASK antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-CASK antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-CASK antibody. Counter stained with hematoxylin.
Fig8: Flow cytometric analysis of HT-29 cells with CASK antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary
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