Anti-NKG2D antibody

Anti-NKG2D antibody

• 概述

• 产品描述Receptor for MICA, MICB, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. Plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. Involved in the immune surveillance exerted by T- and B-lymphocytes.
• 产品名称Anti-NKG2D antibody
• 分子量25 KDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,FC
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human NKG2D 121-219/219
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Membrane; Single-pass type II membrane protein.
• 其它名称KLRK1
  natural killer cell group 2D
  NKG2-D isoform b
  Klrk1
  Nkrp2
  NKG2-D type II integral membrane protein
  NKG2-D-activating NK receptor
  NK cell receptor D
  Killer cell lectin-like receptor subfamily K
  NK lectin-like receptor
  CD314
  CD 314
  CD314 antigen
  D12S2489E
  Killer cell lectin like receptor subfamily K member 1
  Killer cell lectin-like receptor subfamily K member 1
  KLR
  Klrk1
  NKG2 D activating NK receptor
  NKG2 D type II integral membrane protein
  NKG2-D-activating NK receptor
  NKG2D_HUMAN
  NKLLR
  NKR P2
  Nkrp2.
  more

• 应用

  WB:1:500-2000
  FC:1μg/Test

•  

  

  Fig1: Blank control (blue line): Mouse thymus cells (blue).
  Primary Antibody (green line): Rabbit Anti-NKG2D antibody 
  Dilution: 1μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG .
  Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
  Dilution: 1μg /test.
  Protocol
  The cells were fixed with 70% methanol (Overnight at 4℃) . Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

   

  

  Fig2: Sample: Lung (Mouse) Lysate at 40 ug
  Primary: Anti- NKG2Dat 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 25 kD
  Observed band size: 26 kD

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！