Anti-LOX 1 antibody

Anti-LOX 1 antibody

• 概述

• 产品描述Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria.
• 产品名称Anti-LOX 1 antibody
• 分子量31/50 KDa
• 种属反应性Human,Mouse,Rat,Cow,Rabbit
• 验证应用WB,IHC-P,FC
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from rabbit LOX-1 201-273/273
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Cell membrane; Single-pass type II membrane protein. Secreted. Note=A secreted form also exists.
• 其它名称Lectin-type oxidized LDL receptor 1
  OLR1
  lectin-like low density lipoprotein receptor-1
  Oxidized low-density lipoprotein receptor 1
  Ox-LDL receptor 1
  Lectin-like oxidized LDL receptor 1
  Lectin-like oxLDL receptor 1
  LOX-1
  BLOX1
  Contains: Oxidized low-density lipoprotein receptor 1, soluble form A
  Oxidized low-density lipoprotein receptor 1, soluble form B
  OLR1_HUMAN.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:1μg /test

•  

  

  Fig1: Sample: Cerebrum (Rat) Lysate at 40 ug
  Primary: Anti- LOX1at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 31/50 kD
  Observed band size: 50 kD

   

  

  Fig2: Sample:
  293T(Human) Cell Lysate at 30 ug
  Primary: Anti-LOX 1t 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 31/50 kD
  Observed band size: 50 kD

   

  

  Fig3: Positive control: A549 cells
  Concebtration: 1μg/10^6 cells
  Incubation conditions: Avoid light , 30 minutes on the ice.

   

  

  Fig4: Blank control: RSC96 (blue).
  Primary Antibody:Rabbit Anti- LOX 1 antibody Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
  Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
  Protocol
  The cells were fixed with 2% paraformaldehyde (10 min) . Primary antibody 1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed.

   

  

  Fig5: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (LOX 1) Polyclonal Antibody, Unconjugated at 1:500 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

   

  

  Fig6: Paraformaldehyde-fixed, paraffin embedded (Rabbit brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (LOX 1) Polyclonal Antibody, Unconjugatedat 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.

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