Anti-IGFBP4 antibody

Anti-IGFBP4 antibody

• 概述

• 产品描述Insulin-like growth factor-binding protein 4 is a protein that in humans is encoded by the IGFBP4 gene. This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma in both glycosylated and non-glycosylated forms. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. IGFBP-4 is a unique protein and it consistently inhibits several cancer cells in vivo and in vitro. Its inhibitory action has been shown in vivo in prostate and colon. It is secreted by all colon cancer cells. The protein itself does not prevent the formation of cancer. However it may reduce the growth of cancer and act as an apoptotic factor. IGFBP4 has been shown to interact with Insulin-like growth factor 1 and 2.
• 产品名称Anti-IGFBP4 antibody
• 分子量26 KDa
• 种属反应性Human,Mouse,Rat,Chicken,Pig,Cow,Horse,Sheep
• 验证应用WB,IHC-P
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human IGFBP4 181-254/254
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式Affinity purified by Protein A
• 亚细胞定位Secreted.
• 其它名称Insulin-like Growth Factor Binding Protein 4
  BP 4
  BP4
  HT29 IGFBP
  IBP 4
  IBP4
  IGF binding protein 4
  IGFBP 4
  IBP4_HUMAN.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Tissue/cell: Rat spleen tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-IGFBP4 Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody (SP-0023) and DAB (C-0010) staining

   

  

  Fig2: Tissue/cell: Rat adrenal tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-IGFBP4 Polyclonal Antibody, Unconjugated 1:200, overnight at 4℃, followed by conjugation to the secondary antibody (SP-0023) and DAB (C-0010) staining

   

  

  Fig3: Sample:
  Liver (Mouse) Lysate at 40 ug
  Primary: Anti-IGFBP4 at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 26 kDa
  Observed band size: 27 kDa

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！