Anti-LIS1 antibody

Anti-LIS1 antibody

• 概述

• 产品描述Required for proper activation of Rho GTPases and actin polymerization at the leading edge of locomoting cerebellar neurons and postmigratory hippocampal neurons in response to calcium influx triggered via NMDA receptors. Non-catalytic subunit of an acetylhydrolase complex which inactivates platelet-activating factor (PAF) by removing the acetyl group at the SN-2 position (By similarity). Positively regulates the activity of the minus-end directed microtubule motor protein dynein. May enhance dynein-mediated microtubule sliding by targeting dynein to the microtubule plus end. Required for several dynein- and microtubule-dependent processes such as the maintenance of Golgi integrity, the peripheral transport of microtubule fragments and the coupling of the nucleus and centrosome. Required during brain development for the proliferation of neuronal precursors and the migration of newly formed neurons from the ventricular/subventricular zone toward the cortical plate. Neuronal migration involves a process called nucleokinesis, whereby migrating cells extend an anterior process into which the nucleus subsequently translocates. During nucleokinesis dynein at the nuclear surface may translocate the nucleus towards the centrosome by exerting force on centrosomal microtubules. May also play a role in other forms of cell locomotion including the migration of fibroblasts during wound healing.
• 产品名称Anti-LIS1 antibody
• 分子量47 KDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IHC-P
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human LIS1 311-410/410
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome. Cytoplasm, cytoskeleton, spindle (By similarity). Nucleus membrane (Potential). Note=Redistributes to axons during neuronal development. Also localizes to the microtubules of the manchette in e
  
• 其它名称LIS 1
  LIS 2
  LIS-1
  LIS1
  LIS1_HUMAN
  LIS2
  Lissencephaly 1 protein
  Lissencephaly-1 protein
  MDCR
  MDS
  PAF acetylhydrolase 45 kDa subunit
  PAF AH 45 kDa subunit
  PAF AH alpha
  PAF-AH 45 kDa subunit
  PAF-AH alpha
  PAFAH alpha
  PAFAH
  PAFAH1B1
  PAFAHA
  Platelet activating factor acetylhydrolase 1b regulatory subunit 1
  Platelet activating factor acetylhydrolase isoform Ib alpha subunit
  Platelet-activating factor acetylhydrolase IB subunit alpha.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Tissue/cell: Mouse embryos; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-LIS1 Polyclonal Antibody, Unconjugated1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

   

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (LIS1) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (LIS1) Polyclonal Antibody, Unconjugatedat 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig4: Sample:
  Hippocampus (Mouse) Lysate at 40 ug
  Primary: Anti-LISat 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 47 kD
  Observed band size: 49 kD

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