Anti-LIN9 antibody
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概述
- 产品描述Acts as a tumor suppressor. Inhibits DNA synthesis. Its ability to inhibit oncogenic transformation is mediated through its association with RB1. Plays a role in the expression of genes required for the G1/S transition.
- 产品名称Anti-LIN9 antibody
- 分子量62 KDa
- 种属反应性Human,Mouse,Cynomolgus Monkey
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human LIN9
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Nucleus > nucleoplasm. Found in perinucleolar structures. Associated with chromatin.
- 其它名称BARA
Beta subunit-associated regulator of apoptosis
Beta-subunit associated regulator of apoptosis
hLin-9
huLin-9
lin-9
lin9
LIN9_HUMAN
pRB-associated protein
Protein lin-9 homolog
TGS
TUDOR gene similar protein
Type I interferon receptor beta chain-associated protein.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Sample:
Bone (Mouse) Lysate at 40 ug
Primary: Anti-LIN9 at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62 kD
Fig2: Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-LIN9 Polyclonal Antibody, Unconjugated1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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