Anti-Beta-Catenin antibody
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概述
- 产品描述β-Catenin is a key downstream effector in the Wnt signaling pathway. It is implicated in two major biological processes in vertebrates: early embryonic development and tumorigenesis. Phosphorylation at Ser-552 by AMPK promotes stabilizion of the protein, enhancing TCF/LEF-mediated transcription. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
- 产品名称Anti-Beta-Catenin antibody
- 分子量85kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Synthetic peptide (KLH-coupled) witihin human Beta-catenin C terminal.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified
- 亚细胞定位Cytoplasm, Nucleus
- 其它名称Beta catenin antibody
Beta-catenin antibody
Cadherin associated protein antibody
Catenin (cadherin associated protein), beta 1, 88kDa antibody
Catenin beta 1 antibody
Catenin beta-1 antibody
CATNB antibody
CHBCAT antibody
CTNB1_HUMAN antibody
CTNNB antibody
CTNNB1 antibody
DKFZp686D02253 antibody
FLJ25606 antibody
FLJ37923 antibody
OTTHUMP00000162082 antibody
OTTHUMP00000165222 antibody
OTTHUMP00000165223 antibody
OTTHUMP00000209288 antibody
OTTHUMP00000209289 antibody
more
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应用
WB: 1:1,000
IHC-P: 1:200
FC: 1:50-1:100
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Fig1: Western blot analysis of Beta-catenin on different lysates using anti-Beta-catenin antibody at 1/1,000 dilution.
Positive control:
Lane 1: A431
Lane 2: SW480
Lane 3: HCT116
Lane 4: HT-29
Lane 5: Mouse colon
Lane 6: Mouse brain
Fig2: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Beta-catenin antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Beta-catenin antibody. Counter stained with hematoxylin.
Fig4: Flow cytometric analysis of Hela cells with Beta-catenin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
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