Anti-IL-13Ra2 antibody

Anti-IL-13Ra2 antibody

• 概述

• 产品描述Binds as a monomer with high affinity to interleukin-13 (IL13), but not to interleukin-4 (IL4).
• 产品名称Anti-IL-13Ra2 antibody
• 分子量41 KDa
• 种属反应性Human,Mouse,Rat,Dog
• 验证应用WB,FC
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human IL-13Ra2 81-180/380
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Membrane; Single-pass type I membrane protein.
• 其它名称Cancer/testis antigen 19
  CD213a2
  CT19
  I13R2_HUMAN
  IL-13 receptor subunit alpha-2
  IL-13R subunit alpha-2
  IL-13R-alpha-2
  IL-13RA2
  IL13 receptor
  IL13BP
  IL13R
  Il13ra2
  Interleukin 13 binding protein
  Interleukin 13 receptor alpha 2 chain
  Interleukin-13 receptor subunit alpha-2
  Interleukin-13-binding protein.
  more

• 应用

  WB:1:500-2000
  FC:1μg/Test

•  

  

  Fig1: Blank control (Black line): Raji (Black).
  Primary Antibody (green line): Rabbit Anti-IL-13Ra2 antibody 
  Dilution: 1μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG .
  Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE
  Dilution: 1μg /test.
  Protocol
  The cells were fixed with 4% PFA （10min）and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

   

  

  Fig2: Sample:
  Raji(Human) Cell Lysate at 30 ug
  HepG2(Human) Cell Lysate at 30 ug
  Primary: Anti-IL-13Ra2  at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 41 kD
  Observed band size: 41 kD

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！