Anti-ERK1 antibody
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概述
- 产品描述Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines , and research investigators consider it an important target in the diagnosis and treatment of cancer . Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway.
- 产品名称Anti-ERK1 antibody
- 分子量44 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,IHC-P
- 抗体类型兔多抗
- 免疫原peptide
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified
- 亚细胞定位Cytoplasm, nucleus
- 其它名称ERK 1 antibody
ERK antibody
ERK-1 antibody
ERK1 antibody
ERT 2 antibody
ERT2 antibody
Extracellular Signal Regulated Kinase 1 antibody
Extracellular signal related kinase 1 antibody
Extracellular signal-regulated kinase 1 antibody
HGNC6877 antibody
HS44KDAP antibody
HUMKER1A antibody
Insulin Stimulated MAP2 Kinase antibody
Insulin-stimulated MAP2 kinase antibody
MAP kinase 1 antibody
MAP kinase 3 antibody
MAP Kinase antibody
MAP kinase isoform p44 antibody
MAPK 1 antibody
MAPK 3 antibody
MAPK antibody
MAPK1 antibody
Mapk3 antibody
MGC20180 antibody
Microtubule Associated Protein 2 Kinase antibody
Microtubule-associated protein 2 kinase antibody
Mitogen Activated Protein Kinase 3 antibody
Mitogen-activated protein kinase 1 antibody
Mitogen-activated protein kinase 3 antibody
MK03_HUMAN antibody
OTTHUMP00000174538 antibody
OTTHUMP00000174541 antibody
p44 ERK1 antibody
p44 MAPK antibody
p44-ERK1 antibody
p44-MAPK antibody
P44ERK1 antibody
P44MAPK antibody
PRKM 3 antibody
PRKM3 antibody
Protein Kinase Mitogen Activated 3 antibody
more
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应用
WB: 1:500-1:1,000
ICC: 1:200
IHC-P: 1:200
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Fig1: Western blot analysis of ERK1 on different cell lysates using anti- ERK1 antibody at 1/1000 dilution.
Positive control:
Lane 1: Hela
Lane 2: SW480
Lane 3: HCT116
Lane 4: HepG2
Lane 5: PC12
Fig2: ICC staining of ERK1 in LOVO cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-ERK1 antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ERK1 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue using anti-ERK1 antibody. Counter stained with hematoxylin.
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