Anti-NMDAR2B antibody
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概述
- 产品描述NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. In concert with DAPK1 at extrasynaptic sites, acts as a central mediator for stroke damage. Its phosphorylation at Ser-1303 by DAPK1 enhances synaptic NMDA receptor channel activity inducing injurious Ca2+ influx through them, resulting in an irreversible neuronal death.
- 产品名称Anti-NMDAR2B antibody
- 分子量163 KDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,IHC-P,ICC/IF
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human NMDAR2B 451-550/1484
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
- 其它名称NMDA2B (epsilon 2)
GRIN 2B
GRIN2B
hNR 3
hNR3
MGC142178
MGC142180
N methyl D asparate receptor channel subunit epsilon 2
N METHYL D ASPARTATE RECEPTOR CHANNEL SUBUNIT EPSILON 2
N methyl D aspartate receptor subtype 2B
N methyl D aspartate receptor subunit 2B
N methyl D aspartate receptor subunit 3
NMDA NR2B
NMDA R2B
Nmdar2b
NMDE2
NME2
NR2B
NR3
N-Methyl-d-Asprtate receptor 2B
AW490526
Glutamate [NMDA] receptor subunit epsilon 2
Glutamate Receptor Ionotropic N Methyl D Aspartate 2B
Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B
Glutamate receptor ionotropic NMDA2B
Glutamate receptor subunit epsilon 2
Glutamate receptor, ionotropic
NMDE2_HUMAN
glutamate receptor ionotropic, NMDA 2B precursor.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
IF:1:100-500
ICC/IF:1:100-500
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Fig1: Sample: A549 Cell Lysate at 40 ug
Primary: Anti- NMDAR2B at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 163 kD
Observed band size: 210 kD
Fig2: Tissue/cell: rat spinal cord; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NR2B/NMDAR2B Polyclonal Antibody, Unconjugated1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Fig3: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NR2B/NMDAR2B Polyclonal Antibody, Unconjugated1:300, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
