Anti-HMGB1 antibody
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概述
- 产品描述Like the histones, HMGB1, also known as high-mobility group protein 1 (HMG-1) is among the most important chromatin proteins. In the nucleus HMGB1 interacts with nucleosomes, transcription factors, and histones. This nuclear protein organizes the DNA and regulates transcription. After binding, HMGB1 bends DNA, which facilitates the binding of other proteins. HMGB1 is secreted by immune cells through leaderless secretory pathway. Activated macrophages and monocytes secrete HMGB1 as a cytokine mediator of Inflammation. In recent research, HMGB1 has been reported as a novel biomarker for human ovarian cancer.
- 产品名称Anti-HMGB1 antibody
- 分子量25 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原peptide
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified
- 亚细胞定位Nucleus
- 其它名称Amphoterin antibody
Chromosomal protein, nonhistone, HMG1 antibody
DKFZp686A04236 antibody
High mobility group 1 antibody
High mobility group box 1 antibody
High mobility group protein 1 antibody
High mobility group protein B1 antibody
high-mobility group (nonhistone chromosomal) protein 1 antibody
HMG-1 antibody
HMG1 antibody
HMG3 antibody
HMGB 1 antibody
HMGB1 antibody
HMGB1_HUMAN antibody
NONHISTONE CHROMOSOMAL PROTEIN HMG1 antibody
SBP 1 antibody
Sulfoglucuronyl carbohydrate binding protein antibody
more
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应用
WB: 1:500-1:1,000
ICC: 1:200
IHC-P: 1:200
FC: 1:50-1:100
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Fig1: Western blot analysis of HMGB1 on different cell lysates using anti- HMGB1 antibody at 1/500 dilution.
Positive control:
Lane 1: HepG2
Lane 2: MCF-7
Lane 3: F9
Lane 4: A549
Lane 5: PC12
Fig2: ICC staining HMGB1 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining HMGB1 in NIH/3T3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti- HMGB1 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti- HMGB1 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- HMGB1 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human stomach carcinoma tissue using anti- HMGB1 antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- HMGB1 antibody. Counter stained with hematoxylin.
Fig9: Flow cytometric analysis of HMGB1 cells with HMGB1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
