Anti-HK2 antibody [F1-D3]

Anti-HK2 antibody [F1-D3]

• 概述

• 产品描述The hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step of intracellular glucose metabolism, the conversion of glucose to glucose-6-phosphate. Four hexokinase isoenzymes have been identified, including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK III) and hexokinase IV (HXK IV, also designated glucokinase or GCK). Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic cluster is thought to be necessary for membrane binding. This is substantiated by the finding that glucokinase has lower affinity for glucose than do the other hexokinases. HXK I has been shown to be expressed in brain, kidney and heart tissues as well as in hepatoma cell lines. HXK II is involved in the uptake and utilization of glucose by adipose and skeletal tissues. Of the hexokinases, HXK III has the highest affinity for glucose. Glucokinase is expressed in pancreatic beta cells where it functions as a glucose sensor, determining the "set point" for insulin secretion.
• 产品名称Anti-HK2 antibody [F1-D3]
• 分子量102 kDa
• 种属反应性Human
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Mitochondrion outer membrane.
• 其它名称
  • DKFZp686M1669 antibody
  • Hexokinase 2 antibody
  • Hexokinase 2 muscle antibody
  more

• 应用

  WB: 1:500-1:1,000
  IHC-P: 1:100-1:500
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of Hexokinase 2 on different cell lysates using anti- Hexokinase 2 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: Jurkat
  Lane 2: Hela
  Lane 3: HEK293

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded human esophagus cancer tissue using anti- Hexokinase 2 antibody. Counter stained with hematoxylin.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti- Hexokinase 2 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Flow cytometric analysis of K562 cells with Hexokinase 2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).

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