Biotinylated Monoclonal Anti-IFNγ antibody, Human IgG1 (13E6H4)

Biotinylated Monoclonal Anti-IFNγ antibody, Human IgG1 (13E6H4)

表达区间及表达系统（Source）

Anti-IFNγ antibody, Human IgG1 is a chimeric monoclonal antibody recombinantly expressed from HEK293 cells, which combines the variable region of a mouse monoclonal antibody with human IgG1 constant domain. The mouse monoclonal antibody is produced from a hybridoma resulting from fusion of SP2/0 myeloma and B-lymphocytes obtained from a mouse immunized with IFNγ.It is the chemically biotin labeled form of Monoclonal Anti-IFNγ antibody, Human IgG1 Isotype Control .

亚型（Isotype）

Human IgG1/kappa

特异性（Specificity）

This product is a specific antibody specifically reacts with IFN-γ, Human. No cross-reactivity is detected with other human cytokines, including IL-2, IL-4, IL-6, IL-10, GM-CSF and TNF-alpha.

应用说明（Application）

This antibody can be paired with other IFNγ antibodies to detect IFNγ in sandwich ELISA or LFA assay.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

内毒素（Endotoxin）

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20 to -70°C for 12 months in lyophilized state from date of receipt;

2. -70^°C for 3 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

Biotinylated Monoclonal Anti-IFNγ antibody, Human IgG1 (13E6H4) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Biotinylated Monoclonal Anti-IFNγ antibody, Human IgG1 (13E6H4) is more than 90% and the molecular weight of this protein is around 135-155 kDa verified by SEC-MALS.

Report

 

活性（Bioactivity）-ELISA

Immobilized ActiveMax® Human IFN-gamma, Tag Free  can bind Biotinylated Monoclonal Anti-IFNγ antibody, Human IgG1 (13E6H4)  with a linear range of 0.02-50 ng/mL (QC tested).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！